Coding

Part:BBa_K3102016:Design

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-03)


FDH (Formate Dehydrogenase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 903
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence was synthesized and optimized for E.coli on Geneius


Source

This sequence comes from Saccharomyces cerevisiae was found through Yeastgenome: https://www.yeastgenome.org/locus/S000005915

References

Li F, Li YX, Cao YX, Wang L, Liu CG, Shi L, et al. "Modular engineering to increase intracellular NAD(H/+) promotes rate of extracellular electron transfer of Shewanella oneidensis". Nat Commun [Internet]. 2018;9(1):1–13. Available from: http://dx.doi.org/10.1038/s41467-018-05995-8

Zhenquan Lin et al., “Metabolic engineering of Escherichia coli for the production of riboflavin," Microbial Cell Factories, 2014, 13:104.